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sat [2019-08-01 09:55:02]
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sat [2019-08-01 09:55:02]

sulfate adenylyltransferase
Locus
BSU_15590
Isoelectric point
5.65
Molecular weight
42.73 kDa
Protein length
382 aa Sequence Blast
Gene length
1149 bp Sequence Blast
Function
sulfate activation
Product
sulfate adenylyltransferase
Essential
no
E.C.
2.7.7.4
Synonyms
ylnB

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,632,208 1,633,356

The protein

Catalyzed reaction/ biological activity

  • ATP sulfate = diphosphate adenylyl sulfate (according to Swiss-Prot)
  • Protein family

  • sulfate adenylyltransferase family (with YitA, according to UniProt)
  • Paralogous protein(s)

    Structure

  • 1V47 (from Thermus thermophilus, 42% identity) PubMed
  • Additional information

  • belongs to the 100 most abundant proteins PubMed
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • CymR: repression, in CymR regulon
  • S-box: termination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed, in S-box
  • Regulation

  • induced by methionine starvation (S-box) PubMed
  • the S-box RNA is degraded by RNase Y PubMed
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    Biological materials

    Mutant

  • MGNA-B366 (ylnB::erm), available at the NBRP B. subtilis, Japan
  • BKE15590 (sat::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAATATCCATCTCCTCCT, downstream forward: _UP4_GGCGTATCTTAAGGAGGACT
  • BKK15590 (sat::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAATATCCATCTCCTCCT, downstream forward: _UP4_GGCGTATCTTAAGGAGGACT
  • Labs working on this gene/protein

  • [[Isabelle Martin-Verstraete]], Institute Pasteur, Paris, France
  • References

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